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<table WIDTH="90%" BORDER="0" CELLSPACING="2" CELLPADDING="0">

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    <td WIDTH="100%" BGCOLOR="#0000ff"><p ALIGN="RIGHT"><b><font COLOR="#ffffff"

    FACE="Verdana" size="2">MINIATURE OXYGEN SENSOR<br>

    </font><font face="Verdana" color="#00FFFF"><small>(OS 418 : Clark-type O<sub>2</sub>)</small></font></b></td>

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<table border="0" width="95%" cellpadding="5">

  <tr>

    <td width="51%"><font face="Verdana" size="2"><b><span>Electrode Design</span></b></font> <p><font

    face="Verdana" size="1"><span <font face="Verdana" size="1">The OS-418 oxygen sensor is a

    miniature, Clark-type amperometric sensor that is designed for measuring static as well as

    dynamically changing oxygen levels in aqueous and physiologic fluids. <o:p></o:p></span> The oxygen

    sensor consists of a pair of miniaturized electrodes immersed in a drop of aqueous&nbsp;

    KCl electrolyte and separated from the test environment by a replaceable oxygen permeable

    membrane. An outer Ag�/AgCl anode surrounds an internal platinized platinum cathode. The

    5 mil (0.005&quot;) diameter high purity platinum wire cathode is fused into a glass

    sheathing that fills the lumen of the outer silver body. The oxygen permeable membrane is

    placed over the tip of the electrode assembly and secured with an O-ring. With the

    membrane and O-ring in place, the sensor diameter is 4.5 mm (0.177&quot;). The sensor may

    be housed in an black delrin body that exposes only the membrane-covered tip of the sensor

    to your test solution.</o:p></font></td>

    <td BGCOLOR="#e2ebf3" WIDTH="49%"><p class="MsoNormal" style="tab-stops:.25in"><font

    face="Verdana" size="2"><b style="mso-bidi-font-weight:normal"><span>General

    Specifications<o:p></o:p>:</span></b></font></p>

    <table border="0" width="100%">

      <tr>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Sensor Probe

        Length</span></font></td>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">4.182 cm</o:p></span></font></td>

      </tr>

      <tr>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Sensor

        Housing Length</span></font></td>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">2.5 cm<o:p></o:p></span></font></td>

      </tr>

      <tr>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Sensor

        Diameter</span></font></td>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">0.318 cm<o:p></o:p></span></font></td>

      </tr>

      <tr>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Sensor

        Housing Diameter</span></font></td>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">0.787 cm<o:p></o:p></span></font></td>

      </tr>

      <tr>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Connector

        Type</span></font></td>

        <td width="50%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Modified

        Amphenol Sub-Minax P/N 27.9</span></font></td>

      </tr>

    </table>

    <p class="MsoNormal" style="tab-stops:.25in"><font face="Verdana" size="2"><b

    style="mso-bidi-font-weight:normal"><span>Technical Specifications<o:p></o:p>:</span></b></font></p>

    <table border="0" width="100%">

      <tr>

        <td width="67%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Sensor

        Sensitivity</span></font></td>

        <td width="33%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">0 - 150 mm Hg</span></font></td>

      </tr>

      <tr>

        <td width="67%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Response

        Current for Ambient pO<sub>2</sub></span></font></td>

        <td width="33%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">18 nA</span></font></td>

      </tr>

      <tr>

        <td width="67%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Time Constant

        / Response Rate (0 - 63%)</span></font></td>

        <td width="33%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">4 s<o:p></o:p></span></font></td>

      </tr>

      <tr>

        <td width="67%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Bias Voltage

        Required</span></font></td>

        <td width="33%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">-0.7 V<o:p></o:p></span></font></td>

      </tr>

      <tr>

        <td width="67%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">Thermal Drift</span></font></td>

        <td width="33%"><font face="Verdana" size="2"><span

        style="font-family:

Arial;mso-bidi-font-family:&quot;Times New Roman&quot;">4 % / �C<o:p></o:p></span></font></td>

      </tr>

    </table>

    </td>

  </tr>

  <tr>

    <td width="51%"><p align="center"><img src="picts\OS418s.jpg" width="136" height="160"

    alt="OS418s.jpg (2715 bytes)" border="2"></td>

    <td BGCOLOR="#e2ebf3" WIDTH="49%"><font face="Verdana" size="1"><strong>Applications in

    Research and Development</strong></font><ul>

      <li><font face="Verdana" size="1">As an oxygen-level sensor in Micro-BOD (Biological Oxygen

        Demand) measurements.</font></li>

    </ul>

    <ul>

      <li><font face="Verdana" size="1">In monitoring oxygen levels in cell cultures.</font></li>

    </ul>

    <p>&nbsp;</td>

  </tr>

  <tr>

    <td width="51%"><font face="Verdana" size="2"><b>Principle of Operation</b></font><p><font

    face="Verdana" size="1">Using a constant voltage source (potentiostat, OSI-1 or OSI-2), a

    constant -0.7 V bias voltage is applied between both electrodes [cathode (-) and anode

    (+)] of the sensor. In the absence of any other oxidizable species (and there should not

    be any in aq. KCl), this results in a current that is directly proportional to the RATE at

    which molecular oxygen is electrolytically discharged at the cathode. The steady state

    discharge rate is dependent upon diffusion of oxygen across the polymer membrane that

    covers the two electrodes. If all the oxygen that arrives at the cathode is summarily

    discharged, then the discharge rate (current) is directly proportional to the flux (amount

    per unit area per unit time) of oxygen across the membrane. This flux is directly

    proportional to the extra-membrane concentration of dissolved oxygen. In this way the

    observed current is made indirectly proportional to the oxygen concentration.</font></td>

    <td BGCOLOR="#e2ebf3" WIDTH="49%"><font face="Verdana" size="1"><b>The OS 418 Oxygen

    Sensor Package Contains:</b></font><small> </small><p><font face="Verdana" size="1">1

    Oxygen Sensor<br>

    5 reusable O-rings<br>

    1 Sheet of 0.001; Polyethylene Membrane<br>

    1 Membrane Installation Tool (white, cone-shaped) 1 Sensor Housing (two parts) (black

    delrin)<br>

    1 Oxygen Sensor Operations Manual<br>

    </font></td>

  </tr>

  <tr>

    <td width="51%"><p align="center"><img src="picts\OS418c.jpg" width="139" height="95"

    alt="OS418c.jpg (2109 bytes)" border="2"></td>

    <td BGCOLOR="#e2ebf3" WIDTH="49%"><p class="MsoNormal" style="tab-stops:.25in"><font

    face="Verdana" size="1"><strong>Requirements</strong></font><ul>

      <li><font face="Verdana" size="1">Requires Sensor Cable Model SC9BNC (sold separately):

        Model SC9BNC is a flexible coaxial cable with a female Sub-Minax connector on one end (to

        the OS-418 oxygen sensor) and a BNC connector on the other to the OSI-2 instrument.</font></li>

    </ul>

    <ul>

      <li><font face="Verdana" size="1">Requires Oxygen Sensor Instrument Model OSI-1 (single

        channel) or Model OSI-2 (dual channel) (sold separately). Model OSI-1 is a single channel

        oxygen sensor interrogation system for use with the Model OS-418 oxygen sensor.</font></li>

    </ul>

    <ul>

      <li><font face="Verdana" size="1">For best results, the OS-418 requires thermal regulation

        of the test solution and electrode to avoid appreciable thermal drift.</font></li>

    </ul>

    </td>

  </tr>

</table>



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<font SIZE="-1" FACE="Verdana,Arial">



<p align="center"></font><font FACE="Verdana,Arial" size="1">Last Revision 08/12/01; (c)

2000-2001 ABTECH Scientific, Inc.</font></p>



<p align="center"><font FACE="Verdana,Arial" size="1"><b>Contact: Ann M. Wilson, </b>Manager,

<br>

Applications Development ABTECH Scientific, Inc.<br>

911 East Leigh Street G24, Richmond, Virginia 23219<br>

Telephone Number: +1-804-783-7829 Fax Number: +1-804-783-7830<br>

e-mail: <a HREF="mailto:[email protected]">[email protected]</a></font></p>

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